Comparative Analysis of SAF, Protaper Next and BT-Race in Eliminating Enterococcus Faecalis from Long Oval Canals: An Ex Vivo Study

A. Krokidis, C. Bonfanti, A. Cerutti, N. Barabanti, S. Zinelis, P. Panopoulos

Australian Endodontic Journal. 2017. 43(3):110-114.



Comparison of the ability of newly designed rotary files to eliminate viable Enterococcus faecalis populations from long oval root canals of extracted human teeth to that of the self-adjusting file (SAF). One hundred caries-free, single-rooted, long oval teeth were contaminated with E. faecalis. The teeth were randomly distributed into four groups (n = 25) as follows: G.1, manual; G.2, SAF; G.3, ProTaper Next; and G.4, BT-Race. Two microbial samples were obtained from each tooth with sterile paper points, (s1) before and (s2) after instrumentation. The relative reduction in colony-forming units (CFUs) from s1 to s2 measurements was calculated and compared among the groups using parametric Kruskal-Wallis one-way anova on ranks and Dunn's method (a = 0.05). The results indicated a descending order of the groups with regard to efficacy as follows: BT-Race, Next, SAF and manual. The statistical analysis showed that the relative percentage reduction (RR) of CFUs was lower in the manual group than in the other groups, while the SAF group showed a significantly lower RR than the BT-Race group (P < 0.05). The efficacy in reduction of the microbiological load of viable E. faecalis from long oval root canals was different between the tested endodontic systems.

This randomized controlled trial compared the incidence of post-instrumentation pain associated with Mtwo rotary NiTi files and the Self-Adjusting file system following canal shaping and cleaning. 

Additional Info

  • ReDent Nova’s note:

    The "Materials and Methods" paragraph of this article demonstrates how the researchers were unfamiliar with the mode of operation of the Self-Adjusting File: "Shaping proceeded with a pecking motion in an apical direction until a working length was reached". The SAF System was only operated until it reached working length, and not for 4 minutes according to the manufacturer's instructions.

    It is also sad to note that the editors and reviewers of the Australian Endodontic Journal were just as unfamiliar with the SAF System as the authors, that they were not aware of how the researchers did not follow the recommended clinical protocol.

  • publisher: publisher
  • View abstract on PubMed. PMID: 27808450